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Scripps Research Institute

5 ARTICLES PUBLISHED IN JoVE

Medicine

Teratoma Generation in the Testis Capsule

Suzanne E. Peterson¹, Ha T. Tran¹, Ibon Garitaonandia¹, Sangyoon Han¹, Kyle S. Nickey¹, Trevor Leonardo², Louise C. Laurent³, Jeanne F. Loring¹

¹Department of Chemical Physiology, Scripps Research Institute, ²Department of Chemical Physiology, Scripps Research Institute , ³Department of Reproductive Medicine, University of California

Human pluripotent stem cells (hPSCs) have the potential to treat a myriad of different diseases. The utility of these cells lies in the fact that they can differentiate into any cell type in the body. Here we describe the teratoma assay, which is used to demonstrate the pluripotence of hPSCs.

Chemistry

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Sachin S. Shivatare¹, Vidya S. Shivatare¹, Chung-Yi Wu¹, Chi-Huey Wong¹

¹Genomics Research Center, Academia Sinica

A modular approach to the synthesis of N-glycans for attachment to an aluminum oxide-coated glass slide (ACG slide) as a glycan microarray has been developed and its use for the profiling of an HIV broadly neutralizing antibody has been demonstrated.

Immunology and Infection

Antigenic Liposomes for Generation of Disease-specific Antibodies

Kyle J. Bednar^*1, Lakeya Hardy^*2,3, Johanna Smeekens^*3,4, Dharmendra Raghuwanshi⁵, Shiteng Duan⁶, Mike D. Kulis^3,4, Matthew S. Macauley^5,7

¹Janssen R&D, ²Department of Microbiology and Immunology, University of North Carolina, ³UNC Food Allergy Initiative, University of North Carolina, ⁴Department of Pediatrics, University of North Carolina, ⁵Department of Chemistry, University of Alberta, ⁶Department of Molecular Medicine, Scripps Research Institute, ⁷Department of Medical Microbiology and Immunology, University of Alberta

Described is the preparation of antigenic liposomal nanoparticles and their use in stimulating B-cell activation in vitro and in vivo. Consistent and robust antibody responses led to the development of a new peanut allergy model. The protocol for generating antigenic liposomes can be extended to different antigens and immunization models.

Neuroscience

Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity

Jesse Zhan¹, Ruchi Komal¹, William T. Keenan², Samer Hattar¹, Diego C. Fernandez¹

¹Section on Light and Circadian Rhythms (SLCR), National Institute of Mental Health, ²Howard Hughes Medical Institute, Department of Neuroscience, Scripps Research Institute

Here we describe two non-invasive methods to chronically control neuronal activity using chemogenetics in mice. Eye-drops were used to deliver clozapine-N-oxide (CNO) daily. We also describe two methods for prolonged administration of CNO in drinking water. These strategies for chronic neuronal control require minimal intervention reducing animals’ stress.

Chemistry

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Cheng-Wei Cheng¹, Yixuan Zhou¹, Wen-Harn Pan², Supriya Dey³, Chung-Yi Wu¹, Wen-Lian Hsu⁴, Chi-Huey Wong^1,3

¹Genomics Research Center, Academia Sinica, ²Institute of Biomedical Sciences, Academia Sinica, ³Department of Chemistry, Scripps Research Institute, ⁴Institute of Information Science, Academia Sinica

This protocol demonstrates how to use the Auto-CHO software for hierarchical and programmable one-pot synthesis of oligosaccharides. It also describes the general procedure for RRV determination experiments and one-pot glycosylation of SSEA-4.