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University of Magdeburg

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
Martin Wagner 1, Helen Koester 1, Christian Deffge 1, Soenke Weinert 1, Johannes Lauf 1, Alexander Francke 2, Jerry Lee 3, R. C. Braun- Dullaeus 1, Joerg Herold 1
1Department for Cardiology, Angiology and Pneumology, Otto von Guericke University Magdeburg, 2Herzzentrum Dresden, Universitätsklinikum an der Technischen Universität Dresden, Technische Universität Dresden, 3Department of Public Health and Primary Care, University of Cambridge

Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).

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Medicine

Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease
Martin Wagner 1, Claudia Baer 1, Werner Zuschratter 2, Monika Riek-Burchardt 3, Christian Deffge 1, Soenke Weinert 1, Jerry C Lee 4, Ruediger C Braun-Dullaeus 1, Joerg Herold 1
1Department of Cardiology and Angiology, University of Magdeburg, 2Leibniz Institute for Neurobiology, 3Institute of Molecular and Clinical Immunology, University of Magdeburg, 4Division of Hematology/Oncology, Boston Children's Hospital, Harvard Medical School

Monocytes are important mediators of arteriogenesis in the context of peripheral arterial disease. Using a basement membrane-like matrix and intravital microscopy, this protocol investigates monocyte homing and tumor-related angiogenesis after monocyte injection in the femoral artery ligation murine model.

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Behavior

Multi-Modal Signals for Analyzing Pain Responses to Thermal and Electrical Stimuli
Sascha Gruss 1, Mattis Geiger 2, Philipp Werner 3, Oliver Wilhelm 2, Harald C. Traue 1, Ayoub Al-Hamadi 3, Steffen Walter 1
1Department of Medical Psychology, Ulm University, 2Department of Individual Differences and Psychological Assessment, Ulm University, 3Department of Neuro-Information Technology, University of Magdeburg

This article focuses on the experimental elicitation of pain through heat (thermal) and electrical stimulation while recording physiological, visual, and paralinguistic responses. It aims at collecting valid multimodal data for analyzing pain based on its intensity, quality, and duration. 

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