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University of Magdeburg

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

Martin Wagner¹, Helen Koester¹, Christian Deffge¹, Soenke Weinert¹, Johannes Lauf¹, Alexander Francke², Jerry Lee³, R. C. Braun- Dullaeus¹, Joerg Herold¹

¹Department for Cardiology, Angiology and Pneumology, Otto von Guericke University Magdeburg, ²Herzzentrum Dresden, Universitätsklinikum an der Technischen Universität Dresden, Technische Universität Dresden, ³Department of Public Health and Primary Care, University of Cambridge

Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).

Medicine

Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease

Martin Wagner¹, Claudia Baer¹, Werner Zuschratter², Monika Riek-Burchardt³, Christian Deffge¹, Soenke Weinert¹, Jerry C Lee⁴, Ruediger C Braun-Dullaeus¹, Joerg Herold¹

¹Department of Cardiology and Angiology, University of Magdeburg, ²Leibniz Institute for Neurobiology, ³Institute of Molecular and Clinical Immunology, University of Magdeburg, ⁴Division of Hematology/Oncology, Boston Children's Hospital, Harvard Medical School

Monocytes are important mediators of arteriogenesis in the context of peripheral arterial disease. Using a basement membrane-like matrix and intravital microscopy, this protocol investigates monocyte homing and tumor-related angiogenesis after monocyte injection in the femoral artery ligation murine model.

Behavior

Multi-Modal Signals for Analyzing Pain Responses to Thermal and Electrical Stimuli

Sascha Gruss¹, Mattis Geiger², Philipp Werner³, Oliver Wilhelm², Harald C. Traue¹, Ayoub Al-Hamadi³, Steffen Walter¹

¹Department of Medical Psychology, Ulm University, ²Department of Individual Differences and Psychological Assessment, Ulm University, ³Department of Neuro-Information Technology, University of Magdeburg

This article focuses on the experimental elicitation of pain through heat (thermal) and electrical stimulation while recording physiological, visual, and paralinguistic responses. It aims at collecting valid multimodal data for analyzing pain based on its intensity, quality, and duration.