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Appalachian State University

5 ARTICLES PUBLISHED IN JoVE

Medicine

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergström Technique

R. Andrew Shanely^1,2, Kevin A. Zwetsloot², N. Travis Triplett², Mary Pat Meaney^1,2, Gerard E. Farris³, David C. Nieman^1,2

¹Human Performance Laboratory, North Carolina Research Campus, Appalacian State University, ²College of Health Sciences, Appalachian State University, ³Department of Emergency Medicine, Carolinas Medical Center NorthEast

The purpose of this video is to present the modified Bergström skeletal muscle biopsy technique on human subjects.

Immunology and Infection

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood

Mary Pat Meaney¹, David C. Nieman¹, Dru A. Henson², Qi Jiang³, Fu-Zhang Wang³

¹Human Performance Laboratory, North Carolina Research Campus, Appalachian State University, ²Department of Biology, Appalachian State University, ³David H. Murdock Research Institute

The purpose of this manuscript is to present a method for measuring monocyte and granulocyte phagocytosis and oxidative burst activity in human blood samples.

Immunology and Infection

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Jennifer E. McBride¹, Mary P. Meaney², Casey John², David C. Nieman², Renaud F. Warin¹

¹Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, ²Human Performance Laboratory, Appalachian State University, North Carolina Research Campus

This work describes an advanced workflow for the accurate and fast determination of NK (Natural Killer) cell count and NK cell cytotoxicity in human blood samples.

Biology

A Simple and Inexpensive Running Wheel Model for Progressive Resistance Training in Mice

Pieter J. Koopmans^1,2, Kevin A. Zwetsloot^1,2,3

¹Integrative Muscle Physiology Laboratory, Appalachian State University, ²Department of Health and Exercise Science, Appalachian State University, ³Department of Biology, Appalachian State University

This procedure describes a translatable progressive loaded running wheel resistance training model in mice. The primary advantage of this resistance training model is that it is entirely voluntary, thus reducing stress for the animals and the burden on the researcher.

Biology

Profiling the Bacterial Community of Fermenting Traminette Grapes during Wine Production using Metagenomic Amplicon Sequencing

Adrienne Goppold¹, Louis Conradie¹, Otávio Guilherme Gonçalves de Almeida², Elaine Cristina Pereira De Martinis², Folarin Anthony Oguntoyinbo¹

¹A.R. Smith Department of Chemistry and Fermentation Sciences, Appalachian State University, ²Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo

Here, we present a protocol to describe amplicon metagenomic for determining the bacterial community of Traminette grapes, fermenting grapes, and final wine.