Pace University
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Kenric J. Hoegler 1, Martin Distel 2, Reinhard W. Köster 3, John H. Horne 1
1Department of Biology, Pace University, 2Cellular and Molecular Medicine, University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology
The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.
Developmental Biology
Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
Bryan A. Volpe 1, Teresa H. Fotino 1, Aaron B. Steiner 1
1Department of Biology, Pace University
Laser ablation is a widely applicable technology for studying regeneration in biological systems. The presented protocol describes use of a standard laser-scanning confocal microscope for laser ablation and subsequent time-lapse imaging of regenerating interneuromast cells in the zebrafish lateral line.
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