Accedi

Pace University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

Kenric J. Hoegler¹, Martin Distel², Reinhard W. Köster³, John H. Horne¹

¹Department of Biology, Pace University, ²Cellular and Molecular Medicine, University of California, San Diego, ³Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology

The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.

Developmental Biology

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Bryan A. Volpe¹, Teresa H. Fotino¹, Aaron B. Steiner¹

¹Department of Biology, Pace University

Laser ablation is a widely applicable technology for studying regeneration in biological systems. The presented protocol describes use of a standard laser-scanning confocal microscope for laser ablation and subsequent time-lapse imaging of regenerating interneuromast cells in the zebrafish lateral line.