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JoVE Journal

Neuroscience

This content is Open Access.

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

11.9K Views

08:18 min

August 15th, 2011

August 15th, 2011

11,935 Views

0:05

Title

1:07

Transgenic Embryos

1:55

Mounting Embryos

3:37

Micro-injection of DNA Expression Plasmid

4:55

Electroporation

6:00

Mounting Embryos for Imaging

7:03

Expression Targeted to Developing Olfactory Bulb

7:55

Conclusion

The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.

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