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CSIRO

4 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Juan Carlos Polanco¹, Bei Wang¹, Qi Zhou¹, Hun Chy¹, Carmel O'Brien¹, Andrew L. Laslett¹

¹Materials Science and Engineering, CSIRO

We describe the use of the monoclonal antibodies TG30 (CD9) and GCTM-2 for the combined detection of cell surface antigens via fluorescence activated cell sorting (FACS) for the identification and enrichment of live human embryonic stem cells (hESC) using positive selection and also the use of negative selection to purge hESCs from a mixed cell population.

Immunology and Infection

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Xiaoyue Sun^*1,2,3, Xiaoqiang Yang^*1,2,3,4, Chui Zeng^1,2,3, Fayed Attia Koutb Megahed^1,2,3,5, Qi Zhou^1,2,3, Tingdang Liu^1,2,3, Waqas Iqbal^1,2,3, Pingnan Sun^1,2,3, Xiaoling Zhou^1,2,3

¹Stem Cell Research Center, Shantou University Medical College, ²The Center for Reproductive Medicine, Shantou University Medical College, ³Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, ⁴Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, ⁵Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications

This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).

Immunology and Infection

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Qi Zhou^*1,2,3, Xiaoling Xie^*1,2,3, Zhiqian Zhong^1,2,3, Pingnan Sun^1,2,3, Xiaoling Zhou^1,2,3

This manuscript describes a detailed protocol for differentiation of human embryonic stem cells (hESCs) into functional hepatocyte-like cells (HLCs) by continuously supplementing Activin A and CHIR99021 during hESC differentiation into definitive endoderm (DE).

Engineering

Triplet Fusion Upconversion Nanocapsule Synthesis

Tracy H. Schloemer^1,2, Samuel N. Sanders¹, Qi Zhou², Pournima Narayanan³, Manchen Hu², Mahesh K. Gangishetty¹, Daniel Anderson¹, Michael Seitz^1,2, Arynn O. Gallegos², R. Christopher Stokes¹, Daniel N. Congreve^1,2

¹Rowland Institute, Harvard University, ²Department of Electrical Engineering, Stanford University, ³Department of Chemistry, Stanford University

This protocol details the synthesis of upconversion nanocapsules for subsequent use in photopolymerizable resins for triplet fusion upconversion-facilitated volumetric 3D printing.