Diese Studie wurde von der Canadian Institutes of Health Research and Genome Canada unterstützt. JS hält Gehaltszuschüssen Auszeichnungen von der Alberta Heritage Foundation for Medical Research, Heart and Stroke Foundation of Canada und der kanadischen Diabetes Association. Das Labor möchte die technische Unterstützung von Oroboros Instruments bei der Akquisition der Saponin-permeabilisierten Faser-Technik anerkennen.

<ol>
	<li>Saks, V. A., Veksler, V. I., Kuznetsov, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Permeabilized+cell+and+skinned+fiber+techniques+in+studies+of+mitochondrial+function+in+vivo.">Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo.</a> Mol Cell Biochem. 184, 81-100 (1998).</li><li>Gnaiger, E., Kuznetsov, A. V., Schneeberger, S., Heldmaier, G., Klingenspor, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondria+in+the+cold.">Mitochondria in the cold.</a> Life in the Cold. , 431-442 (2000).</li><li>Rasmussen, H. N., Rasmussen, U. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oxygen+solubilities+of+media+used+in+electrochemical+respiration+measurements.">Oxygen solubilities of media used in electrochemical respiration measurements.</a> Anal Biochem. 319, 105-113 (2003).</li><li>Visscher, G. D. e., Rooker, S., Jorens, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pentobarbital+fails+to+reduce+cerebral+oxygen+consumption+early+after+non-hemorrhagic+closed+head+injury+in+rats.">Pentobarbital fails to reduce cerebral oxygen consumption early after non-hemorrhagic closed head injury in rats.</a> J Neurotrauma. 22, 793-806 (2005).</li><li>Kuznetsov, A. V., Veksler, V., Gellerich, F. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+mitochondrial+function+in+situ+in+permeabilized+muscle+fibers,+tissues+and+cells.">Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells.</a> Nat Protoc. 3, 965-976 (2008).</li><li>Gnaiger, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oxygen+conformance+of+cellular+respiration.+A+perspective+of+mitochondrial+physiology.">Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology.</a> Adv Exp Med Biol. 543, 39-55 (2003).</li><li>Gnaiger, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capacity+of+oxidative+phosphorylation+in+human+skeletal+muscle:+new+perspectives+of+mitochondrial+physiology.">Capacity of oxidative phosphorylation in human skeletal muscle: new perspectives of mitochondrial physiology.</a> Int J Biochem Cell Biol. 41, 1837-1845 .</li><li>Sena, S., Hu, P., Zhang, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impaired+insulin+signaling+accelerates+cardiac+mitochondrial+dysfunction+after+myocardial+infarction.">Impaired insulin signaling accelerates cardiac mitochondrial dysfunction after myocardial infarction.</a> J Mol Cell Cardiol. 46, 910-918 (2009).</li><li>Boudina, S., Sena, S., O'Neill, B. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+mitochondrial+oxidative+capacity+and+increased+mitochondrial+uncoupling+impair+myocardial+energetics+in+obesity.">Reduced mitochondrial oxidative capacity and increased mitochondrial uncoupling impair myocardial energetics in obesity.</a> Circulation. 112, 2686-2695 (2005).</li><li>Lenaz, G., Genova, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Structure+and+organization+of+mitochondrial+respiratory+complexes:+a+new+understanding+of+an+old+subject.">Structure and organization of mitochondrial respiratory complexes: a new understanding of an old subject.</a> Antioxid Redox Signal. 12, 961-1008 .</li><li>Lemieux, H., Hoppel, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondria+in+the+human+heart.">Mitochondria in the human heart.</a> J Bioenerg Biomembr. 41, 99-106 (2009).</li><li>O, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retarded+diffusion+of+ADP+in+cardiomyocytes:+possible+role+of+mitochondrial+outer+membrane+and+creatine+kinase+in+cellular+regulation+of+oxidative+phosphorylation.">Retarded diffusion of ADP in cardiomyocytes: possible role of mitochondrial outer membrane and creatine kinase in cellular regulation of oxidative phosphorylation.</a> Biochim Biophys Acta. 1144, 134-148 (1993).</li><li>Endo, M., Kitazawa, T., Morad, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=E-C+coupling+studies+in+skinned+cardiac+fibers.">E-C coupling studies in skinned cardiac fibers.</a> Biophysical Aspects of Cardiac Muscle. , 307-327 (1978).</li><li>Veksler, V. I., Kuznetsov, A. V., Sharov, V. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondrial+respiratory+parameters+in+cardiac+tissue:+a+novel+method+of+assessment+by+using+saponin-skinned+fibers.">Mitochondrial respiratory parameters in cardiac tissue: a novel method of assessment by using saponin-skinned fibers.</a> Biochim Biophys Acta. 892, 191-196 (1987).</li><li>Bangham, A. D., Horne, R. W., Glauert, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Action+of+saponin+on+biological+cell+membranes.">Action of saponin on biological cell membranes.</a> Nature. , 196-952 (1962).</li><li>Daum, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lipids+of+mitochondria.">Lipids of mitochondria.</a> Biochim Biophys Acta. 822, 1-42 (1985).</li><li>Milner, D. J., Mavroidis, M., Weisleder, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Desmin+cytoskeleton+linked+to+muscle+mitochondrial+distribution+and+respiratory+function.">Desmin cytoskeleton linked to muscle mitochondrial distribution and respiratory function.</a> J Cell Biol. 150, 1283-1298 (2000).</li><li>Skladal, D., Sperl, W., Schranzhofer, R., Skladal, E., Gellerich, F., Wyss, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preservation+of+mitochondrial+functions+in+human+skeletal+muscle+during+storage+in+high+energy+preservation+solution+(HEPS).">Preservation of mitochondrial functions in human skeletal muscle during storage in high energy preservation solution (HEPS).</a> What is Controlling Life?. , 268-271 (1994).</li><li>Kuznetsov, A. V., Wiedemann, F. R., Winkler, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+saponin-permeabilized+muscle+fibers+for+the+diagnosis+of+mitochondrial+diseases.">Use of saponin-permeabilized muscle fibers for the diagnosis of mitochondrial diseases.</a> Biofactors. 7, 221-223 (1998).</li><li>Gnaiger, E., Dykens, J., Will, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polarographic+oxygen+sensors,+the+oxygraph+and+high-resolution+respirometry+to+assess+mitochondrial+function.">Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function.</a> Drug-Induced Mitochondrial Dysfunction. , 327-352 (2008).</li></ol>

Die Saponin-permeabilisierten Herz-Faser-Technik bietet eine einzigartige Kompromiss zwischen in vitro und in vivo Beurteilung der mitochondrialen OXPHOS Sauerstoffverbrauch. Vorteile dieser Technik sind physiologische Relevanz im Vergleich zu isolierten Mitochondrien als zelluläre Architektur erhöht wird beibehalten. Während der Plasmamembran abgebaut wird, intrazellulären Membranstrukturen wie Mitochondrien 12, 14, sarkoplasmatischen Retikulum 14, bleiben Myofilamente und da...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>100% Ethanol</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>HC600</td>
<td>&nbsp;</td>
<tr>
<td>70% Ethanol</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>HC-1000</td>
<td>&nbsp;</td>
<tr>
<td>Adenosine 5&#x2032;-diphosphate monopotassium salt dihydrate (ADP)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>A5285</td>
<td>&nbsp;</td>
<tr>
<td>Albumin from bovine serum essentially fatty acid&#x2013;free</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>A-6003</td>
<td>&nbsp;</td>
<tr>
<td>Antimycin A</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>A8674</td>
<td>&nbsp;</td>
<tr>
<td>Ascobic acid</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>A4403</td>
<td>&nbsp;</td>
<tr>
<td>Adenosine 5&#x2032;-triphosphate disodium salt hydrate (ATP)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>A2383</td>
<td>&nbsp;</td>
<tr>
<td>Atractyloside</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>A6882</td>
<td>&nbsp;</td>
<tr>
<td>Calcium carbonate</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>C4830</td>
<td>&nbsp;</td>
<tr>
<td>Carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>C2920</td>
<td>&nbsp;</td>
<tr>
<td>Cytochrome c</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>C7752</td>
<td>&nbsp;</td>
<tr>
<td>Digitonin</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>D141</td>
<td>&nbsp;</td>
<tr>
<td>Dithiothreitol</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>D9779</td>
<td>&nbsp;</td>
<tr>
<td>Ethylene glycol-bis-(2-aminoethylether)-N,N,N&#x2032;,N&#x2032;-tetraacetic acid (EGTA)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>E4378</td>
<td>&nbsp;</td>
<tr>
<td>Glutamic acid</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>27647</td>
<td>&nbsp;</td>
<tr>
<td>HEPES</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>H4034</td>
<td>&nbsp;</td>
<tr>
<td>Imidazole</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>I5513</td>
<td>&nbsp;</td>
<tr>
<td>Ketamine</td>
<td>&nbsp;</td>
<td>Pfizer</td>
<td>&nbsp;</td>
<td>Ketaset</td>
</tr>
<tr>
<td>Lactobionic acid</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>153516</td>
<td>&nbsp;</td>
<tr>
<td>Magnesium chloride (MgCl2)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>M9272</td>
<td>&nbsp;</td>
<tr>
<td>Magnesium chloride hexahydrate (MgCl2&#x2219;6H2O)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>M9272</td>
<td>&nbsp;</td>
<tr>
<td>Malic acid</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>M1000</td>
<td>&nbsp;</td>
<tr>
<td>MES</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>M3671</td>
<td>&nbsp;</td>
<tr>
<td>N,N,N&#x2019;,N&#x2019;-Tetramethyl- pphenylenediamine Dihydrochloride (TMPD)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>T3134</td>
<td>&nbsp;</td>
<tr>
<td>Oligomycin</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>O4876</td>
<td>&nbsp;</td>
<tr>
<td>Phosphocreatine</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>P7936</td>
<td>&nbsp;</td>
<tr>
<td>Potassium Chloride</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>P9541</td>
<td>&nbsp;</td>
<tr>
<td>Potassium Hydroxide</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>P5958</td>
<td>&nbsp;</td>
<tr>
<td>Potassium cyanide</td>
<td>&nbsp;</td>
<td>Fluka</td>
<td>60178</td>
<td>&nbsp;</td>
<tr>
<td>Potassium phosphate monobasic</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>P5655</td>
<td>&nbsp;</td>
<tr>
<td>Rotenone</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>R8875</td>
<td>&nbsp;</td>
<tr>
<td>Saponin</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>47036</td>
<td>&nbsp;</td>
<tr>
<td>Sodium Pentobarbital</td>
<td>&nbsp;</td>
<td>Ceva Sante Animale</td>
<td>1715 138</td>
<td>Conc. 54.7 mg/ml</td>
</tr>
<tr>
<td>Sodium pyruvate</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>P2256</td>
<td>&nbsp;</td>
<tr>
<td>Succinic acid</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>S3674</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>S7903</td>
<td>&nbsp;</td>
<tr>
<td>Taurine</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>T8691</td>
<td>&nbsp;</td>
<tr>
<td>Xylazine</td>
<td>&nbsp;</td>
<td>Bayer</td>
<td>&nbsp;</td>
<td>Rompun</td>
</tr>
<tr>
<td>ddH2O</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Ice</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Oroboros Oxygraph-2k</td>
<td>&nbsp;</td>
<td>Oroboros Instruments</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Kimwipes</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>21905-026</td>
<td>&nbsp;</td>
<tr>
<td>15ml polypropylene centrifuge tubes</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>89004-368</td>
<td>&nbsp;</td>
<tr>
<td>50ml polypropylene centrifuge tubes</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>89004-364</td>
<td>&nbsp;</td>
<tr>
<td>Straight Jewelers Forceps</td>
<td>&nbsp;</td>
<td>George Tiemann &amp; Co.</td>
<td>160-50B</td>
<td>&nbsp;</td>
<tr>
<td>Curved Jewelers Forceps</td>
<td>&nbsp;</td>
<td>George Tiemann &amp; Co.</td>
<td>160-57B</td>
<td>&nbsp;</td>
<tr>
<td>Straight Surgery Scissors</td>
<td>&nbsp;</td>
<td>George Tiemann &amp; Co.</td>
<td>105-402</td>
<td>&nbsp;</td>
<tr>
<td>Sterile Surgical Blade</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>BD371610</td>
<td>&nbsp;</td>
<tr>
<td>0.45-&mu;m Syringe filters</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>CA28145-485</td>
<td>&nbsp;</td>
<tr>
<td>pH meter</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>CA11388-308</td>
<td>&nbsp;</td>
<tr>
<td>Glass Petri dishes</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>89000-300</td>
<td>&nbsp;</td>
<tr>
<td>12-well Polystyrene Tissue Culture Plates</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>82050-926</td>
<td>&nbsp;</td>
<tr>
<td>Plate Stirrer</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>97042-594</td>
<td>&nbsp;</td>
<tr>
<td>Fisherbrand Microbars</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>14-511-67</td>
<td>&nbsp;</td>
<tr>
<td>Weigh Scale</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>CA11278-162</td>
<td>&nbsp;</td>
<tr>
<td>10&mu;l Hamilton Micro Syringe</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>14-815-1</td>
<td>&nbsp;</td>
<tr>
<td>25&#x03BC;l Hamilton Micro Syringe</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>14-824-7</td>
<td>&nbsp;</td>
<tr>
<td>50&mu;l Hamilton Micro Syringe</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>14-824-5</td>
<td>&nbsp;</td>
<tr>
<td>Nalgene Squeeze Bottles</td>
<td>&nbsp;</td>
<td>Wilkem Scientific</td>
<td>LNA2407-1000</td>
<td>&nbsp;</td>
<tr>
<td>Polystyrene Weighing Dishes</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>89106-750</td>
<td>&nbsp;</td>
<tr>
<td>Dissecting Microscope</td>
<td>&nbsp;</td>
<td>Olympus</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

respirometric oxidative phosphorylation assessment in saponin-permeabilized cardiac fibers

Untersuchung der Funktion der Mitochondrien ist ein wichtiger Parameter der kardialen Physiologie als Mitochondrien im Energiestoffwechsel, oxidativen Stress, Apoptose, dem Altern, mitochondriale Enzephalomyopathien und Toxizität von Medikamenten beteiligt sind. Vor diesem Hintergrund werden Technologien zur kardialen Funktion der Mitochondrien Maßnahme verlangen. Eine Technik, die einen integrativen Ansatz verwendet, um die Funktion der Mitochondrien Maßnahme ist respirometrische oxidative Phosphorylierung (OXPHOS) Analyse. Das Prinzip der respirometrische OXPHOS Beurteilung ist um die Messung der Sauerstoffkonzentration unter Verwendung einer Clark-Elektrode zentriert. Da die permeabilisierten Faserbündel verbraucht Sauerstoff, Sauerstoff-Konzentration in der geschlossenen Kammer abnimmt. Anhand ausgewählter Substrat-Inhibitor-Entkoppler Titration Protokolle, werden Elektronen auf bestimmten Seiten der Elektronen-Transportkette vorgesehen, der die Auswertung der mitochondrialen Funktion. Vor respirometrische Analyse der mitochondrialen Funktion, mechanische und chemische vorbereitende Techniken werden eingesetzt, um das Sarkolemm der Muskelfasern permeabilisieren. Chemische Permeabilisierung beschäftigt Saponin, um selektiv zu perforieren die Zellmembran unter Beibehaltung zellulären Architektur. Dieses Papier beschreibt ausführlich die Schritte bei der Vorbereitung Saponin-Haut Herz-Fasern für den Sauerstoffverbrauch Messungen der mitochondrialen OXPHOS bewerten beteiligt. Darüber hinaus Tipps zur Fehlerbehebung sowie spezifische Substrate, Inhibitoren und Entkuppler, die zur Mitochondrien-Funktion an bestimmten Standorten der Elektronen-Transportkette zu bestimmen sein kann. Wichtig ist, können die beschriebenen Protokoll leicht zu Herz-und Skelett-Gewebe von verschiedenen Tiermodellen und humanen Proben angewendet werden.

Watch this Scientific Journal Video about Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers at JoVE.com

Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers

Saponin-permeabilisierten Faseraufbereitung in Verbindung mit respirometrische oxidative Phosphorylierung Analyse bietet integrative Beurteilung der Funktion der Mitochondrien. Mitochondriale Atmung in physiologischen und pathologischen Zuständen kann reflektieren verschiedene regulatorische Einflüsse einschließlich mitochondrialer Interaktionen, Morphologie und Biochemie.

Respirometrische Oxidative Phosphorylierung Assessment in Saponin-permeabilisierten Cardiac Fibers

Investigation of mitochondrial function represents an important parameter of cardiac physiology as mitochondria are involved in energy metabolism, ...

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24.5K Aufrufe.  University of Calgary. Saponin-permeabilisierten Faseraufbereitung in Verbindung mit respirometrische oxidative Phosphorylierung Analyse bietet integrative Beurteilung der Funktion der Mitochondrien. Mitochondriale Atmung in physiologischen und pathologischen Zuständen kann reflektieren verschiedene regulatorische Einflüsse einschließlich mitochondrialer Interaktionen, Morphologie und Biochemie.