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JoVE Journal

Neuroscience

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Analysis of Dendritic Spine Morphology in Cultured CNS Neurons

34.8K Views

11:48 min

July 13th, 2011

July 13th, 2011

34,784 Views

0:05

Title

2:00

Transfection of Primary Cultured Cortical Neurons

3:14

Treatment of Primary Cultured Cortical Neurons Grown on 18 mm Coverslips

3:46

Fixation and Immunocytohistochemistry

5:21

Quantitative Analysis of Spine Morphologies

6:58

Time-lapse Imaging

9:43

Representative Results: Analysis of Dendritic Spine Morphology

11:04

Conclusion

Numerous recent studies have identified mutations in synaptic proteins associated with brain pathologies. Primary cultured cortical neurons offer great flexibility in examining the effects of these disease-associated proteins on dendritic spine morphology and motility.

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