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JoVE Journal

Biology

Author Produced

This content is Open Access.

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

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18:45 min

September 2nd, 2012

September 2nd, 2012

24,574 Views

0:00

Title

0:29

Materials and Equipment

2:12

Identifying Wells With Crystals

3:13

Opening the Well

4:04

"Closeup": Opening Wells with Cubic Phase - Method I

6:38

"Closeup": Opening Wells with Cubic Phase - Method 2

8:27

Opening Well with Sponge Phase - Unsuccessfully

10:25

Opening Well with Sponge Phase - Successfully

14:20

Harvesting Crystals

15:16

Harvesting Crystals from the Cubic Phase

16:12

Harvesting Crystals from the Sponge Phase

16:40

Cryo-Cooling Crystals

17:25

Storage/Transport Dewars

17:42

Crystal Images

18:03

Concluding Remarks

Herein is described procedures implemented in the Caffrey Membrane Structural and Functional Biology Group to harvest and cryo-cool membrane protein crystals grown in lipidic cubic and sponge phases for use in structure determination using macromolecular X-ray crystallography.

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