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13:10 min
October 14th, 2013
DOI :
10.3791/50664-v
Kapitel
0:05
Title
1:13
Preparation of Frozen Nematode Slides
4:17
Preparation of Slides of Individual Nematodes
5:32
Fixation: Light Fix of Methanol-Acetone
6:30
Fixation: Hard Fix Using Formaldehyde or Glutaraldehyde
8:22
Preparation of Slides for Microscopy
10:32
Representative Images
12:45
Conclusion
Summary
"Freeze-cracking," a method for exposing the inner tissues of the nematode C. elegans to antibodies for protein localization, is demonstrated.
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