Transposon-vermittelte Integration von Plasmid-DNA in die Subventrikularzone von neugeborenen Mäusen zu Novel Modelle von Glioblastoma generieren

Here we describe an efficient and versatile protocol to induce, monitor and analyze novel glioblastomas (GBM) using transposon DNA injected into the ventricles of neonatal mice. Cells of the subventricular zone, which take up the plasmid, transform, proliferate and generate tumors with histo-pathological characteristics of human GBM.