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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

DOI :

10.3791/57609-v

October 7th, 2018

October 7th, 2018

9,329 Views

1UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM, Sorbonne Universités, 2UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition, Sorbonne Universités

Here, we present a protocol to promote transgene integration and production of founder transgenic mice with high efficacy by a simple injection of a lentiviral vector in the perivitelline space of a fertilized oocyte.

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Keywords Lentiviral

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