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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

DOI :

10.3791/54067-v

11:37 min

June 11th, 2016

June 11th, 2016

16,693 Views

1Microbiology, School of Natural Sciences, National University of Ireland Galway, 2School of Biological Sciences, University of Essex

A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.

Tags

Simultaneous DNA RNA Extraction

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