Calibrating the Deflection Sensitivity and Spring Constant of the AFM Probe
6:28
Measuring the Elastic Modulus on Mouse Arteries Ex Vivo
7:50
Data Analysis
8:47
Results: Determining Aortic Stiffness
9:29
Conclusion
Transkript
The overall goal of this protocol is to describe the procedures for using Atomic Force Microscopy to measure the Ex Vivo elastic modulus of mouse aortas. This method help answer key questions in the vascular biomechanics and disease field such as
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We present detailed protocols for isolation of aortas from mouse and measurement of their elastic modulus using atomic force microscopy.