Visualizing Stromules with Confocal Fluorescence Microscopy and Image Processing
3:28
Extracting Intact Chloroplasts to Visualize Stromule Dynamics
5:58
Results: Quantifying Stromule Frequency in N. benthamiana Leaves
7:22
Conclusion
Transkript
The overall goal of this procedure is to visualize and determine stromule frequency using live cell confocal fluorescence microscopy within leaves, and for visualizing stromules in vitro using chloroplast extracted from leaves. These experimental
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Protocols to investigate the dynamics of chloroplast stromules, the stroma-filled tubules that extend from the surface of chloroplasts, are described.