Isolation of Neurospheres from the Adult Mouse Brain
2:43
Embedding and Sectioning of Neurospheres for immunostaining
4:10
Starvation and Analysis of Cilia
6:01
Results: Starvation Induces Ciliogenesis in Dissociated, Adherent Neutral Stem/Progenitor Cells
7:19
Conclusion
Transkript
The overall goal of this procedure is to utilize neurosphere based culturing methods for primary neural stem and progenitor cells to study primary cilium biogenesis and trafficking. This method can help answer key questions in the primary cilia fi
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The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.