Isolation of Alveolar Macrophages (AMs) from Murine Bronchoalveolar Lavage (BAL) Fluid
3:58
Isolation of AMs from Mouse Lung: Single-cell Suspension
6:04
In Vitro Culture of AMs
7:06
Results: Characteristics of Mouse Alveolar Macrophages
7:42
Conclusion
Transkript
The overall goal of this procedure is to isolate human and mice alveolar macrophages from lungs and bronchoalveolar lavage fluid and develop the method for their in vitro culture. This method can help answer some key questions in the field of pulm
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This communication describes methodologies for isolation and culture of alveolar macrophages from humans and murine models for experimental purposes.