Murine Kidney Microdissection for Renal Segment Isolation
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Results: Characterizing the Renal Segments
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Conclusion
Transkript
The overall goal of this microscopic dissection procedure is to isolate discrete segments of living renal tissue with intact renal architecture for tissue engineering applications. This method can help answer key questions in the field of tissue e
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Tissue-engineered renal constructs provide a solution for the organ shortage and deleterious effects of dialysis. Here, we describe a protocol to micro dissect murine kidneys for isolation of cortico-medullary segments. These segments are implanted into scaffold-free cellular constructs, forming renal organoids.