Woodboring beetle their body surface are always hard in SEM. The Tween-20 is added into fixative and washing solution. The body surface of woodboring beetle In TEM, the Tween-20 can enhance fixative penetration into the body wall and better fix ti
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In order to observe ultrastructure of insect sensilla, scanning and transmission electron microscopy (SEM and TEM, respectively) sample preparation protocol were presented in the study. Tween 20 was added into the fixative to avoid sample deformation in SEM. Fluorescence microscopy was helpful for improving slicing accuracy in TEM.