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JoVE Journal

Cancer Research

This content is Free Access.

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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08:18 min

April 7th, 2023

April 7th, 2023

1,299 Views

0:04

Introduction

1:01

Oligonucleotide‐Conjugated Antibody Staining

4:39

Multiplexed Imaging Reporter Plate

5:16

Operating the Multiplexed Imaging Machine

6:31

Multiplex Image Collection and Analysis

7:01

Results: Visualization of the Acquired Multiplexed Images

7:28

Conclusion

Transkript

Multiplexed barcoding image analysis has recently improved the characterization of the tumor microenvironment, permitting comprehensive studies of cell composition, functional state, and cell-cell interactions. Herein, we describe a staining and imaging protocol using the barcoding of oligonucleotide-conjugated antibodies and cycle imaging, which allows for the use of a high-dimensional image analysis technique.

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