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Sacred Heart University

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex
Maya Frankfurt 1,2, Rachel Bowman 2
1Department of Science Education, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, 2Department of Psychology, Sacred Heart University

The protocol describes a modification of the rapid Golgi method, which can be adapted to any part of the nervous system, for staining neurons in the hippocampus and medial prefrontal cortex of the rat.

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Biology

Laser Microdissection for Species-Agnostic Single-Tissue Applications
Alyssa Woronik 1,2, Karin Kiontke 1, Raya S. Jallad 1, R. Antonio Herrera 3, David H. A. Fitch 1
1Center for Developmental Genetics, New York University, 2Sacred Heart University, 3Baylor School

A protocol is described that uses laser microdissection to isolate individual nematode tissues for RNA-sequencing. The protocol does not require species-specific genetic toolkits, allowing gene expression profiles to be compared between different species at the level of single-tissue samples.

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