Sacred Heart University
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex
Maya Frankfurt 1,2, Rachel Bowman 2
1Department of Science Education, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, 2Department of Psychology, Sacred Heart University
The protocol describes a modification of the rapid Golgi method, which can be adapted to any part of the nervous system, for staining neurons in the hippocampus and medial prefrontal cortex of the rat.
Biology
Laser Microdissection for Species-Agnostic Single-Tissue Applications
Alyssa Woronik 1,2, Karin Kiontke 1, Raya S. Jallad 1, R. Antonio Herrera 3, David H. A. Fitch 1
1Center for Developmental Genetics, New York University, 2Sacred Heart University, 3Baylor School
A protocol is described that uses laser microdissection to isolate individual nematode tissues for RNA-sequencing. The protocol does not require species-specific genetic toolkits, allowing gene expression profiles to be compared between different species at the level of single-tissue samples.
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