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Sacred Heart University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex

¹Department of Science Education, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, ²Department of Psychology, Sacred Heart University

The protocol describes a modification of the rapid Golgi method, which can be adapted to any part of the nervous system, for staining neurons in the hippocampus and medial prefrontal cortex of the rat.

Biology

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Alyssa Woronik^1,2, Karin Kiontke¹, Raya S. Jallad¹, R. Antonio Herrera³, David H. A. Fitch¹

¹Center for Developmental Genetics, New York University, ²Sacred Heart University, ³Baylor School

A protocol is described that uses laser microdissection to isolate individual nematode tissues for RNA-sequencing. The protocol does not require species-specific genetic toolkits, allowing gene expression profiles to be compared between different species at the level of single-tissue samples.