JoVE Content Production

Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.

This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.

This video demonstrates the protocol for isolating and culturing explants of the mouse forebrain from embyonic day 12 mice. Procedures for removal of the uterus, embryos from uterus, and dissection of embryos are given. In addition the methodology for transferring these explants onto specialized membranes on which they are cultured is demonstrated. The development of the forebrain can be studied in vitro using this preparations as well as changes in gene expression.

This video describes the method used for isolation of neuroprecursors from the developing cortex of embryonic mice. The procedure for removing embryos from the uterus, dissecting the cortical tissue, and digesting the isolated cerebral cortex is shown.

Back in 1905, in what is now the Czech Republic, Eduard Zirm performed the first corneal transplantation surgery (keratoplasty), which restored vision to a patient blinded by corneal injury. Today, eye banks all over the world prepare, store, and distribute donated corneas to hospitals so that thousands of sight-saving keratoplasties can be performed every year. In June 2012, JoVE has its eye on two research groups, one from Italy and the other from Michigan, who demonstrate two distinct methods for corneal graft preparation prior to transplantation.

Historically, JoVE, The Journal of Visualized Experiments, has focused primarily on biomedical research and has developed subsections for Bioengineering, Clinical and Translational Medicine, Immunology and Infection, and Neuroscience. This July, JoVE launches its Applied Physics section, which includes a range of content from Plasma Physics to Materials Science. We begin the new section with a notable article from Purdue University, where researchers in the Center for Laser-Based Manufacturing are studying.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

This September in JoVE, researchers from the School of Medicine at the Free University of Berlin demonstrate a novel method for studying how stroke patients compensate for visual field defects. To do this, our authors make use of a driving simulator complete with brakes, a steering wheel, and turn signals. Using driving simulation software and sophisticated eye tracking, researchers can compare the gaze behavior of stroke patients as they navigate through virtual driving courses with varying degrees of complexity. Though posterior cerebral artery infarction can lead to similar visual deficits in patients, some are able to navigate through the driving courses by developing compensatory eye movements, while others crash into dangerous obstacles, like wild boars. Through the analysis of compensatory gaze behavior employed by patients, our authors see great potential for using driving simulation as a tool to rehabilitate stroke patients trying to overcome the blind spots in their visual fields.

Here are some highlights from the October 2012 Issue of Journal of Visualized Experiments (JoVE).

In this issue, Oestreicher et al. show us how to isolate magnetotactic bacteria from freshwater samples, and concentrate the bacteria at one end of a glass capillary. The magnetotactic bacteria can then be visualized by light and transmission electron microscopy, and used for various other assays.

Here are some highlights from the December 2012 Issue of Journal of Visualized Experiments (JoVE).

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

Here's a look at what's coming up in the February 2013 Issue of Journal of Visualized Experiments (JoVE).

Here are some highlights from the March 2013 issue of Journal of Visualized Experiments (JoVE).

Here are some highlights from the April 2013 Issue of Journal of Visualized Experiments (JoVE).

Here are some highlights from the May 2013 Issue of Journal of Visualized Experiments (JoVE).

Here are some highlights from the June 2013 Issue of Journal of Visualized Experiments (JoVE).

Here are some highlights from the July 2013 Issue of Journal of Visualized Experiments (JoVE).

January 2015: This Month in JoVE - Introducing JoVE Developmental Biology

February 2015: This Month in JoVE - Tracking Down Foodborne Illness, Imaging Baby Brains, and Paying Scientific Attention to Attention

March 2015: This Month in JoVE - Solving Crime with Science, Applying Technology to Understand Trees, and Studying Protein Synthesis on a Chip

April 2015: This Month in JoVE - Studying Locomotion in Drunken Worms, Preserving Human Liver for Transplantation, and Visualizing Bacterial Swarms

This Month in JoVE - Assessing Freezing Tolerance in Plants, Patterning 2D Shapes with DNA, Studying Ischemia In Vitro, Studying Social Cognition in Monkeys

June 2015: This Month in JoVE - Celebrating JoVE's 100th Issue

July 2015 - This Month in JoVE: Treating Canine Halitosis, Minimizing Workplace Stress, and Assessing Electrical Activity and Herbicide Resistance in Plants

August 2015 - This Month in JoVE: Isolating Stem Cells, Bioengineering the Kidney, and Getting Kids to eat Carrots

September 2015 - This Month in JoVE: Measuring Greenhouse Gases and Herbicide Resistance, Bioengineering Bone, and Analyzing Neuromuscular Control with Virtual Reality

October 2015 - This Month in JoVE: tuberculosis infection modeling, telemetric temperature pills, bioengineered cartilage, and 3D neuronal networks

November 2015 - This Month in JoVE: Drosophila Social Space, Structured Rehabilitation for Multifunctional Prosthetics, and Thermal Imaging in Wild Birds

December 2015 - This Month in JoVE: Flu Surveillance in Swine, DNA Nanorobots, Analyzing Crude Oil, and a Translational Model of Depression

JoVE - Year in Review: 2015

February 2016 - This Month in JoVE: Photoconvertible Proteins, Gold Nanoparticles, PET Principles, and Bone Marrow Microenvironments

March 2016 - This Month in JoVE: RNA interference in Mosquitoes, iPSCs Derived from Nasal Epithelia, Air Sampling of Atmospheric Aerosols, and Autologous Micro-grafts for Skin Lesions

April 2016 - This Month in JoVE: Cell Migration, Bacterial Motility, Psycholinguistics, and In Vitro Eye Model for Contact Lenses

May 2016 - This Month in JoVE: Cheetah footprints, lens stiffness, and digitally printed solar cells

June 2016 - This Month in JoVE: Blueberry Analysis, Impact Testing Football Helmets, Stencil Micropatterning of Stem Cells, and a Test for Soil Plasticity

July 2016: This Month in JoVE

August 2016 - This Month in JoVE: Sampling in Suspension Feeders, Staining Three-Dimensional Skin, Spasms in the Heart Vasculature, and Emotional Reponses to Beverages

September 2016-This Month in JoVE: Introducing JoVE Genetics, JoVE Biochemistry, and JoVE Cancer Research

October 2016: This Month in JoVE

November 2016 - This Month in JoVE: Turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates

December 2016: This Month in JoVE

2016: This Year in JoVE

JoVE Monthly Highlights: February 2017

JoVE Monthly Highlights: March 2017

JoVE Monthly Highlights: April 2017

JoVE Monthly Highlights: May 2017

JoVE Monthly Highlights: August 2017

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JoVE Monthly Highlights: October 2017

JoVE Monthly Highlights: November 2017

JoVE Monthly Highlights December 2017

JoVE Monthly Highlights: January 2018

JoVE Monthly Highlights: February 2018

JoVE Monthly Highlights: March 2018

JoVE Monthly Highlights: April 2018

JoVE Monthly Highlights: May 2018

JoVE Monthly Highlights: June 2018

JoVE Monthly Highlights: July 2018

JoVE Monthly Highlights: August 2018

JoVE Monthly Highlights: September 2018

JoVE Monthly Highlights: October 2018

JoVE Monthly Highlights: November 2018

JoVE Monthly Highlights: December 2018

JoVE Monthly Highlights: January 2019

JoVE Monthly Highlights: February 2019

JoVE Monthly Highlights: April 2019

Journal Monthly Highlights: May 2019

Journal Monthly Highlights: June 2019

Journal Monthly Highlights: July 2019

Journal Monthly Highlights: August 2019

Journal Monthly Highlights: September 2019

Journal Monthly Highlights: October 2019

Journal Monthly Highlights: November 2019

Journal Monthly Highlights: December 2019