<a href="https://www.jove.com/video/4313">Intraoperatoria detección de endometriosis sutil: un nuevo paradigma para la detección y tratamiento del dolor pélvico asociado con la pérdida de la integridad Peritoneal</a> Bruce A. Lessey 1, H. Lee Higdon III 1, Sara E. Miller 2, Thomas A. Price 3 1 Departamento de Obstetricia y Ginecología, División de Endocrinología Reproductiva e Infertilidad, Greenville Hospital System, 2 Departamento de Patología de la Duke University Health System, 3 Departamento de Obstetricia y Ginecología, División de Endocrinología Reproductiva e Infertilidad de la Universidad Duke La pérdida de integridad peritoneal proporciona un nuevo paradigma para entender y tratar el dolor pélvico crónico en mujeres con formas leves de endometriosis y puede ser fácilmente detectado usando la instilación intraoperatoria de colorante en el momento de la laparoscopia. Aire táctil y Análisis de Movimiento de estrategias de muestreo antenales en las abejas melíferas (Apis mellifera L.)</a> Samir Mujagic, Simon Michael Würth, Sven Hellbach, Volker Dürr Cibernética Biológica, CITEC - Tecnología - Cognitive Interaction Center of Excellence, Universidad de Bielefeld En este protocolo se muestra cómo acondicionar aprovechado las abejas a los estímulos táctiles e introducir una técnica de captura de movimiento 2D para analizar la cinemática de escala fina patrón de muestreo antenal. <a href="https://www.jove.com/video/4320">Una preparación in vitro para provocar y grabación de programas de alimentación del motor con los movimientos fisiológicos en Aplysia californica</a> Jeffrey M. McManus 1, Hui Lu 1, Hillel Chiel J. 1, 2, 3 1 Departamento de Biología de la Universidad Case Western Reserve, 2 Departamento de Neurociencias de la Case Western Reserve University, 3 Departamento de Ingeniería Biomédica de la Case Western Reserve University Se describe una técnica para registrar extracelularmente y estimular los nervios, músculos y neuronas individuales identificados in vitro tiempo que se obtiene y la observación de los diferentes tipos de comportamientos de alimentación en el aparato de alimentación de Aplysia. <a href="https://www.jove.com/video/50081">Generación y Recuperación de β-células esferoides de PEG-péptido de crecimiento Paso hidrogeles</a> Asad Raza, Chien-Chi Lin Departamento de Ingeniería Biomédica, Purdue Escuela de Ingeniería y Tecnología, Universidad de Indiana - Purdue University en Indianápolis El siguiente protocolo proporciona técnicas para encapsular las células β del páncreas en el paso-Crecimiento peptídicos PEG-hidrogeles formados por tiol-eno clic en la foto-reacciones. Esta plataforma de material no sólo ofrece un microambiente citocompatible para encapsulación de células, sino que permite también controlada por el usuario rápida recuperación de las estructuras celulares formadas dentro de los hidrogeles. <a href="https://www.jove.com/video/4400">Replicación del cromosoma Timing Combinado con la hibridación fluorescente in situ</a> Leslie Smith, Mateo Thayer Departamento de Bioquímica y Biología Molecular, Knight Cancer Institute, Oregon Health &amp; Science University Un método cuantitativo para el análisis de sincronización cromosoma replicación se describe. El método utiliza la incorporación de BrdU en combinación con hibridación in situ fluorescente (FISH) para evaluar el tiempo de replicación de los cromosomas de mamíferos. Esta técnica permite la comparación directa de los cromosomas reordenados y poco reorganizado dentro de the misma célula. <a href="https://www.jove.com/video/4392">El insecto Galleria mellonella como un modelo de infección poderosa para investigar la patogénesis bacteriana</a> Nalini Ramarao, Christina Nielsen-Leroux, Didier Lereclus INRA, Micalis UMR1319, Francia Haemocolic infección intra oral y de las larvas de la polilla mayor de la cera Galleria mellonella se describe. Este insecto puede ser usado para estudiar los factores de virulencia de entomopatógeno así como mamíferos bacterias oportunistas. La cría de los insectos, los métodos de infección y ejemplos de análisis in vivo se describen.

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december 2012: this month in jove

Éstos son algunos aspectos destacados de la 12 2012 cuestión de la revista Journal of experimentos visualizados (Jove). Este mes, Smith y Thayer combinar dos métodos clásicos-bromodesoxiuridina (BrdU) incorporación y fluorescencia i n hibridación in situ (FISH)-para examinar el tiempo de replicación de los cromosomas individuales mamíferos. Al analizar las diferencias en la incorporación de BrdU, los autores pueden detectar reordenamientos cromosómicos cómo hacer que la replicación asíncrona entre cromosomas homólogos de una sola célula. En JoVE Neuroscience, McManus et al. Estudiar la actividad neuromuscular en la babosa de mar de California (Aplysia californica), y los movimientos de su aparato de alimentación durante la alimentación. Nuestros autores nos muestran cómo aislar partes de la boca de la babosa, inducir programas de alimentación (tales como morder y tragar, y analizar la actividad de los nervios, los músculos y las neuronas individuales durante la alimentación. t &quot;&gt; También en JoVE Neuroscience, Mujagic et al. tomar las abejas (Apis mellifera) de la colmena al laboratorio, donde permiten a las abejas para explorar diferentes superficies metálicas con sus antenas. Luego, los investigadores alimentar al agua con azúcar abejas después de cierto estímulos táctiles. Esto entrena a las abejas a extender sus probóscide, en previsión de agua con azúcar, cuando sus superficies de contacto antenas particulares. Nuestros autores luego analizar cómo los cambios en el movimiento antenal corresponden al aprendizaje asociativo táctil o no asociativas. En Bioingeniería JoVE, Raza y Lin demostrar un procedimiento para la generación de encapsulados β-esferoides de células en un hidrogel de polímero reticulado. Después de recuperar los agregados, los autores pueden utilizar los esferoides de células β para estudiar las células y los tejidos a base de terapias para la investigación en medicina regenerativa. En Medicina Clínica y Traslacional JoVE, Lessey et al. Utilizar colorante azul de metileno para identificar soncomo la endometriosis, una condición que causa dolor pélvico y otros problemas genitourinarios en las mujeres. Por manchar las superficies peritoneales con azul de metileno, nuestros autores pueden identificar áreas de endometriosis sutil que de otro modo podrían perderse durante la laparoscopia. Esto permite a nuestros autores a eliminar todo el tejido afectado, lo que mejora los resultados quirúrgicos en pacientes con endometriosis. En JoVE Inmunología e Infección, nos enteramos de que las polillas de cera (Galleria mellonella), también conocido como nido de abeja, las polillas tienen mucho en común con nosotros los mamíferos. De hecho, las polillas tienen complejos sistemas inmunes innatos que pueden ser utilizados para caracterizar los factores de virulencia microbiana que son relevantes para infecciones en mamíferos. Ramarao et al. Nos muestran cómo criar las larvas y las infectan con microbios o toxinas microbianas para los ensayos de patogenia. Esta vista previa resume tan sólo unos pocos notables en video artículos en el 12 2012 cuestión de Jove. Para la versión de larga duracións de estos y muchos más artículos en video, por favor visite <a href="http://www.jove.com">www.jove.com</a> .

Watch this Scientific Journal Video about December 2012: This Month in JoVE at JoVE.com

December 2012: This Month in JoVE

Éstos son algunos aspectos destacados de la 12 2012 cuestión de la revista Journal of experimentos visualizados (Jove).

Diciembre de 2012: Este mes en la JoVE

Here are some highlights from the December 2012 issue of Journal of Visualized Experiments (JoVE).
This month, Smith and Thayer combine two classical ...

Research

JoVE Journal

Biology

8.4K vistas.  Massachusetts Eye and Ear. Éstos son algunos aspectos destacados de la 12 2012 cuestión de la revista Journal of experimentos visualizados (Jove).

Video: December 2012: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

08 2012: Este mes en Jove

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Investigación

Biología

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Un año en revisión

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...