<a href="https://www.jove.com/video/4189">LabVIEW operado Novela Osmometer nanolitros de Investigaciones de hielo de fijación con proteínas</a> Ido Braslavsky 1, 2, Ran Drori 1 1 Instituto de Bioquímica, Bromatología y Nutrición, The Robert H. Smith Facultad de Agricultura, Alimentación y Medio Ambiente, de la Universidad Hebrea de Jerusalén, 2 Departamento de Física y Astronomía de la Universidad de Ohio Proteínas de unión de hielo (IBP), también conocidas como proteínas anticongelantes, inhibir el crecimiento del hielo y son un aditivo prometedor para su uso en la crioconservación de tejidos. La principal herramienta utilizada para investigar los IBP es el osmómetro nanolitros. Hemos desarrollado un hogar diseñado etapa de enfriamiento montado en un microscopio óptico y se controla utilizando una rutina de costumbre-construido LabVIEW. El osmómetro nanoliter descrito aquí manipulado la temperatura de la muestra de una manera ultra-sensible. <ahref = &quot;https://www.jove.com/video/50022&quot;&gt; Origami Inspirado autoensamblaje de partículas modeladas y Reconfigurable Shivendra Pandey 1 de Evin Gultepe 1, David H. Gracias 1, 2 1 Departamento de Ingeniería Química y Biomolecular, The Johns Hopkins University, 2 Departamento de Química, The Johns Hopkins University Se describen detalles experimentales de la síntesis de partículas modeladas y reconfigurable de dos dimensiones (2D) precursores. Esta metodología se puede utilizar para crear las partículas en una variedad de formas, incluyendo poliedros y dispositivos de agarre en escalas de longitud que van desde el micro a escala de centímetros. <a href="https://www.jove.com/video/50225">Rapid ensayos colorimétricos para distinguir cualitativamente el ARN y el ADN en muestras Biomolecular</a> Jennifer Patterson, Cameron Mura Departamiento de Química de la Universidad de Virginia Un conjunto de ensayos colorimétricos se describe para la proteína rápidamente distintivo, ARN, ADN, y ducing re-azúcares en muestras de biomoléculas potencialmente heterogéneos. <a href="https://www.jove.com/video/4323">Preparación y uso de samario diyoduro (SMI 2) en Síntesis Orgánica: La función mecánica de HMPA y Ni (II) sales en la reacción de Barbier Samario</a> Dhandapani V. Sadasivam, Kimberly A. Choquette, Robert A. Flores II Departamento de Química de la Universidad de Lehigh Un procedimiento sencillo para la preparación de samario diyoduro (SMI 2) en THF se describe. El papel de los dos aditivos principales, a saber hexametilfosforamida (HMPA) y Ni (acac) 2 en las reacciones mediadas Sm se demuestra en la reacción de Sm-Barbier. <a href="https://www.jove.com/video/50021">Trayendo</a>El universo visible en Focus con Robo-AO Christoph Baranec 1, 2, Riddle Reed 1, Nicholas Law M. 3, AN Ramaprakash 4, Shriharsh P. Tendulkar 2, Khanh Bui 1, Mahesh P. Burse 4, Pravin Chordia 4, Hillol K. Das 4, TC Jack Davis 1 , Richard G. Dekany 1, Mansi M. Kasliwal 5, Shrinivas R. Kulkarni 1, 2, Timothy D. Morton 2, O. Eran Ofek 6, Sujit Punnadi 4 1 Caltech ópticos Observatorios, California Institute of Technology, 2 Departamento de Astronomía de California Institute of Technology, 3 Dunlap Instituto de Astronomía y Astrofísica de la Universidad de Toronto, 4 Centro Interuniversitario para Astronomía y Astrofísica, 5 Observatorios de la Institución Carnegie para la Ciencia , 6 Benoziyo Center for Astrophysics, Weizmann<html> Instituto de Ciencia La luz de los objetos astronómicos deben viajar a través de la turbulenta atmósfera de la Tierra antes de que pueda ser captado por los telescopios terrestres. Para habilitar la proyección de imagen directa en máxima resolución angular teórica, técnicas avanzadas, como las empleadas por el Robo-AO de óptica adaptativa del sistema debe ser utilizado. <a href="https://www.jove.com/video/50130">Datos de análisis de trayectoria para peatones Actividad Estudio espacio-tiempo</a> Feng Qi 1, Du Fei 2 1 Escuela de Medio Ambiente y Ciencias Biológicas, Universidad de Kean, 2 Departamento de Geografía de la Universidad de Wisconsin-Madison Un conjunto de métodos de procesamiento espaciotemporales se presentan para analizar los datos de trayectoria humanos, tales como la recogida usando un dispositivo GPS, con el fin de modelar peatonales de espacio-tiempo actividades. / Video/4416 &quot;&gt; La ecocardiografía fetal murino Gene H. Kim Departamento de Medicina de la Sección de Cardiología, Instituto de Investigación Cardiovascular de la Universidad de Chicago La muerte fetal y perinatal es una característica común en el estudio de las alteraciones genéticas que afectan el desarrollo cardíaco. Alta frecuencia de imágenes por ultrasonido ha mejorado en 2-D y resolución pueden proporcionar información relevante sobre el desarrollo cardíaco temprano y es un método ideal para detectar el impacto en la estructura y función cardíaca antes de la muerte.

No hay conflictos de interés declarado.

february 2013: this month in jove

He aquí un vistazo a lo que viene en el 02 2013 cuestión de la revista Journal of experimentos visualizados (Jove). Desde el primer número de Jove, la investigación biomédica ha compuesto la fracción predominante de nuestro contenido, y tenemos secciones para la Neurociencia, Medicina Clínica y Traslacional, Bioingeniería e Inmunología. En julio de 2012, que se ramificó a las ciencias físicas con el lanzamiento de JoVE Física Aplicada. Este mes, estamos contentos de haber lanzado el primer vídeo-artículos en Química Jove, que cuenta con temas que van desde la bioquímica compleja, para el desarrollo del ensayo, a la ingeniería química y la síntesis orgánica. En el departamento de ingeniería química, Pandey et al. Combinar litografía plana con auto-plegado métodos para crear tres dimensiones de estructuras inspiradas en el arte del origami. A través de la exposición a campos térmicos o magnéticos, estas microestructuras pueden ser inducidas a doblarse sobre objetos, y puede be utiliza para extirpar tejido - una aplicación que podría traducirse en la toma de biopsias nanoescala en vivo. Pasando a la bioquímica, y Braslavsky Drori presentar un experimento diseñado para medir los efectos de hielo proteínas de unión sobre la formación de cristales de hielo. A través de microscopía de vídeo y el análisis subsiguiente de vídeo, térmica histéresis-la diferencia entre las temperaturas de congelación y fusión se pueden determinar en la presencia de proteínas anticongelantes, que protegen muchos organismos como bacterias y plantas de temperaturas de congelación. En JoVE Física Aplicada, Baranec et al. apuntar a las estrellas en nuestro artículo de astronomía muy en primer lugar, con lo que el universo visible en el enfoque con el Robo-AO sistema láser de óptica adaptativa. Con la ayuda de un rayo láser de alta potencia, este sistema corrige las ondas de luz entrantes que se distorsionan por la turbulencia atmosférica lo que los objetos en el cielo puede verse con alta resolución. Los autores planean utilizar esta técnicaQue para encontrar planetas fuera de nuestro Sistema Solar, y hacer observaciones precisas de los fenómenos astronómicos. Mirando hacia atrás en la Tierra, Qi y Du examinar cómo los movimientos humanos en las dimensiones espaciales y temporales se puede utilizar para estudiar la transmisión de enfermedades. Habilitados para GPS dispositivos se pueden utilizar para recopilar automáticamente datos humanos trayectoria, que el proceso autores mediante un interfaz interactivo novela. Las trayectorias también se puede animar en Google Earth. Estos métodos pueden ayudar a detectar los &quot;puntos calientes&quot; para la transmisión de enfermedades infecciosas, como la gripe. En Medicina Clínica y Traslacional JoVE, Kim presenta un método no invasivo para los corazones de imagen de ratón fetal en el útero mediante ecografía de alta frecuencia. Este procedimiento, también llamado ecocardiografía, puede ser usado para estudiar la estructura y la función normal del corazón, así como los mecanismos de la enfermedad cardiovascular. Incluso se puede utilizar para el diagnóstico precoz de defectos en el corazón, haciendo de este un potenteherramienta con muchas aplicaciones científicas y clínicas. A partir de la visualización de los confines más lejanos del espacio, hasta el análisis de los movimientos humanos en el espacio y el tiempo, que acaba alcanzó a ver el nuevo contenido de Júpiter en febrero de 2013. Al igual que el universo, seguimos creciendo, y este mes marca nuestro destino en una nueva frontera con el lanzamiento de la Química en JoVE: El diario de experimentos visualizados.

Watch this Scientific Journal Video about February 2013: This Month in JoVE at JoVE.com

February 2013: This Month in JoVE

He aquí un vistazo a lo que viene en el 02 2013 cuestión de la revista Journal of experimentos visualizados (Jove).

Febrero 2013: Este mes en la JoVE

Here's a look at what's coming up in the February 2013 issue of Journal of Visualized Experiments (JoVE).
Since JoVE's first issue, biomedical research ...

Research

JoVE Journal

Biology

8.9K vistas.  Massachusetts Eye and Ear. He aquí un vistazo a lo que viene en el 02 2013 cuestión de la revista Journal of experimentos visualizados (Jove).

Video: February 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

08 2012: Este mes en Jove

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Investigación

Biología

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Un año en revisión

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...