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University of Toledo College of Medicine and Life Sciences

9 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
Daxiang Li 1, Jian Wu 1, Yan Bai 1, Xiaochen Zhao 2, Lijun Liu 1
1Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, 2Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences

We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.

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Immunology and Infection

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
Yi Yao 1,2, Yun Liu 1,3, Akira Takashima 1
1Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, 2Department of Internal Medicine, Yale University School of Medicine, 3Department of Pathophysiology, Southern Medical University (China)

This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

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Cancer Research

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
Phillip Huyett 1, Mark Gilbert 1, Lijun Liu 2, Robert L. Ferris 2, Seungwon Kim 1
1Department of Otolaryngology, University of Pittsburgh Medical Center, 2University of Pittsburgh Cancer Institute, Hillman Cancer Center

Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.

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Biology

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
Jia-Hsin Huang 1, Yun Liu 1, Yu-Hsien Lin 1,2,3, Xavier Belles 4, How-Jing Lee 1
1Department of Entomology, National Taiwan University, 2Biology Centre, Institute of Entomology, Czech Academy of Sciences, 3Faculty of Science, University of South Bohemia, 4Institute of Evolutionary Biology, CSIC-UPF

This manuscript demonstrates the depletion of gene expression in the midgut of the German cockroach through oral ingestion of double-stranded RNA encapsulated in liposomes.

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Medicine

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes
Yun Liu *1, Yin Liang *1, Mengxue Wang *1, Chen Wang 1, Heng Wei 2, Keiji Naruse 1, Ken Takahashi 1
1Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.

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Behavior

Tactile Vibrating Toolkit and Driving Simulation Platform for Driving-Related Research
Ao Zhu 1, Annebella Tsz Ho Choi 1, Ko-Hsuan Ma 1, Shi Cao 2, Han Yao 1, Jian Wu 3, Jibo He 1
1Psychology Department, School of Social Sciences, Tsinghua University, 2Department of Systems Design Engineering, University of Waterloo, 3Haier Innovation Design Center, Haier Company

This protocol describes a driving simulation platform and a tactile vibrating toolkit for the investigation of driving-related research. An exemplar experiment exploring the effectiveness of tactile warnings is also presented. 

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Behavior

An Assessment Method and Toolkit to Evaluate Keyboard Design on Smartphones
Yincheng Wang 1, Ke Wang 1, Yuqi Huang 1, Di Wu 2, Jian Wu 3, Jibo He 1
1Department of Psychology, School of Social Sciences, Tsinghua University, 2Department of Computer Science, Beijing Normal University, 3Haier Innovation Design Center, Haier Company

The presented protocol integrates various evaluation methods and demonstrates a method to evaluate the keyboard design on smartphones. Pairs matched by English characters are proposed as the input material, and the transition time between two keys is used as the dependent variable.

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Biology

Surgically Induced Cardiac Volume Overload by Aortic Regurgitation in Mouse
Jian Wu *1, Shijun Wang *1, Le Kang *1, Chunjie Yang *1, Jianguo Jia 1, Xingxu Wang 2, Ge Wei 1, Xuan Li 1, Jieyun You 2, Yunzeng Zou 1
1Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes of Biomedical Sciences, Fudan University, 2Department of Cardiovascular Medicine, Shanghai East Hospital, School of Medicine, Tongji University

This protocol presents a practical guide on the surgery for creation of aortic regurgitation (AR) in the mouse. Assessment of the AR mouse by echocardiography and invasive hemodynamic measurement recapitulates its clinically relevant characteristics of volume overload-induced eccentric hypertrophy, suggesting its promising application in the study of cardiac hypertrophy.

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Biology

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle
Yan Bai 1, Xingbin Ai 1
1Division of Newborn Medicine, Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School

The present protocol describes preparing and utilizing mouse precision-cut lung slices to assess the airway and intrapulmonary arterial smooth muscle contractility in a nearly in vivo milieu.

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