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University of Toledo College of Medicine and Life Sciences

9 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy

Daxiang Li¹, Jian Wu¹, Yan Bai¹, Xiaochen Zhao², Lijun Liu¹

¹Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, ²Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences

We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.

Immunology and Infection

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Yi Yao^1,2, Yun Liu^1,3, Akira Takashima¹

¹Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, ²Department of Internal Medicine, Yale University School of Medicine, ³Department of Pathophysiology, Southern Medical University (China)

This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

Cancer Research

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

Phillip Huyett¹, Mark Gilbert¹, Lijun Liu², Robert L. Ferris², Seungwon Kim¹

¹Department of Otolaryngology, University of Pittsburgh Medical Center, ²University of Pittsburgh Cancer Institute, Hillman Cancer Center

Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.

Biology

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Jia-Hsin Huang¹, Yun Liu¹, Yu-Hsien Lin^1,2,3, Xavier Belles⁴, How-Jing Lee¹

¹Department of Entomology, National Taiwan University, ²Biology Centre, Institute of Entomology, Czech Academy of Sciences, ³Faculty of Science, University of South Bohemia, ⁴Institute of Evolutionary Biology, CSIC-UPF

This manuscript demonstrates the depletion of gene expression in the midgut of the German cockroach through oral ingestion of double-stranded RNA encapsulated in liposomes.

Medicine

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

Yun Liu^*1, Yin Liang^*1, Mengxue Wang^*1, Chen Wang¹, Heng Wei², Keiji Naruse¹, Ken Takahashi¹

¹Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, ²Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.

Behavior

Tactile Vibrating Toolkit and Driving Simulation Platform for Driving-Related Research

Ao Zhu¹, Annebella Tsz Ho Choi¹, Ko-Hsuan Ma¹, Shi Cao², Han Yao¹, Jian Wu³, Jibo He¹

¹Psychology Department, School of Social Sciences, Tsinghua University, ²Department of Systems Design Engineering, University of Waterloo, ³Haier Innovation Design Center, Haier Company

This protocol describes a driving simulation platform and a tactile vibrating toolkit for the investigation of driving-related research. An exemplar experiment exploring the effectiveness of tactile warnings is also presented.

Behavior

An Assessment Method and Toolkit to Evaluate Keyboard Design on Smartphones

Yincheng Wang¹, Ke Wang¹, Yuqi Huang¹, Di Wu², Jian Wu³, Jibo He¹

¹Department of Psychology, School of Social Sciences, Tsinghua University, ²Department of Computer Science, Beijing Normal University, ³Haier Innovation Design Center, Haier Company

The presented protocol integrates various evaluation methods and demonstrates a method to evaluate the keyboard design on smartphones. Pairs matched by English characters are proposed as the input material, and the transition time between two keys is used as the dependent variable.

Biology

Surgically Induced Cardiac Volume Overload by Aortic Regurgitation in Mouse

Jian Wu^*1, Shijun Wang^*1, Le Kang^*1, Chunjie Yang^*1, Jianguo Jia¹, Xingxu Wang², Ge Wei¹, Xuan Li¹, Jieyun You², Yunzeng Zou¹

¹Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes of Biomedical Sciences, Fudan University, ²Department of Cardiovascular Medicine, Shanghai East Hospital, School of Medicine, Tongji University

This protocol presents a practical guide on the surgery for creation of aortic regurgitation (AR) in the mouse. Assessment of the AR mouse by echocardiography and invasive hemodynamic measurement recapitulates its clinically relevant characteristics of volume overload-induced eccentric hypertrophy, suggesting its promising application in the study of cardiac hypertrophy.

Biology

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle

Yan Bai¹, Xingbin Ai¹

¹Division of Newborn Medicine, Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School

The present protocol describes preparing and utilizing mouse precision-cut lung slices to assess the airway and intrapulmonary arterial smooth muscle contractility in a nearly in vivo milieu.