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Southwest Jiaotong University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Shammy Raj¹, Lei Lu², Larry D. Unsworth¹

¹Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering, University of Alberta, ²School of Life Science and Engineering, Southwest Jiaotong University

Techniques for generating a library of short peptides that can activate mast cells via the MRGPRX2 receptor are described. Associated techniques are easy, inexpensive, and can be extended to other cell receptors.

Chemistry

Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

Dan Fang¹, Rui Wang¹, Xinyi Yu¹, Yuan Tian¹

¹Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University

This protocol describes the synthesis of cyclic cell-penetrating peptides with aromatic cross-links and the evaluation of their permeability across biological barriers.