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the Fifth Affiliated Hospital of Guangzhou Medical University

11 ARTICLES PUBLISHED IN JoVE

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JoVE Core

Fabrication and Operation of a Nano-Optical Conveyor Belt
Jason Ryan 1, Yuxin Zheng 1, Paul Hansen 1, Lambertus Hesselink 2
1Electrical Engineering, Stanford University, 2Applied Physics, Stanford University

The scalability and resolution of conventional optical manipulation techniques are limited by diffraction. We circumvent the diffraction limit and describe a method of optically transporting nanoparticles across a chip using a gold surface patterned with a path of closely spaced C-shaped plasmonic resonators.

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Developmental Biology

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
Jiayin Yang *1,2,6, Lai-Yung Wong *2, Xiao-Yu Tian *3, Rui Wei 1,2, Wing-Hon Lai 2, Ka-Wing Au 2, Zhiwei Luo 4,5, Carl Ward 4,5, Wai-In Ho 2, David P. Ibañez 4,5, Hao Liu 4,5, Xichen Bao 4,5, Baoming Qin 4,5, Yu Huang 3, Miguel A. Esteban 4,5,7, Hung-Fat Tse 1,2,6,7
1Department of Medicine, University of Hong Kong-Shenzhen Hospital, 2The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, 3School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, 4Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, 5Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 6Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, 7Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia.

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Medicine

The Lower Body Positive Pressure Treadmill for Knee Osteoarthritis Rehabilitation
Junjie Liang *1,2, Yuanyuan Guo *1,2, Yuxin Zheng 1,2, Shijuan Lang 1, Hongxin Chen 1,2, Yaoyao You 1,2, Bryan O’Young 3,4, Haining Ou *1,2, Qiang Lin *1,2
1Department of Rehabilitation, The Fifth Affiliated Hospital of Guangzhou Medical University, 2Experiment Education Model Center of Rehabilitation Medicine, Guangzhou Medical University, 3Department of Physical Medicine and Rehabilitation, Geisinger Health System, 4Department of Rehabilitation Medicine, New York University School of Medicine

Here, based on a clinician’s point-of-view, we propose a two-model lower body positive pressure (LBPP) protocol (walking and squatting models) in addition to a clinical, functional assessment methodology, including details for further encouragement of the development of non-drug surgical intervention strategies in knee osteoarthritis patients. However, we only present the effect of LBPP training in improvement of pain and knee function in one patient through three-dimensional gait analysis. The exact, long-term effects of this approach should be explored in future studies.

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Behavior

Quantitative Static and Dynamic Assessment of Balance Control in Stroke Patients
Qiang Lin *1,2,3,4, Yuxin Zheng *1,2,3,4, Peixi Lian 3,4, Yuanyuan Guo 1,3,4, Haojie Huang 5, Zhiwei Luo 1,2, Wanying Li 1,2, Hongxin Chen 1,2,3,4, Sufen Li 1, Shijuan Lang 1,2,4, Yumei Chen 6, Haining Ou *1,2,3,4, Junjie Liang *1,2,3,4
1Department of Rehabilitation, The Fifth Affiliated Hospital of Guangzhou Medical University, 2The Rehabilitation Medicine Lab of Guangzhou Medical University, 3Experiment Education Model Center of Rehabilitation Medicine, Guangzhou Medical University, 4Department of Rehabilitation Therapy, Guangzhou Medical University, 5College of Physical Education and Sports, Beijing Normal University, 6Department of Rehabilitation, Nanfang Hospital, Southern Medical University

Presented here is a quantitative, clinical balance assessment method suitable for stroke patients with balance disorders.

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Behavior

Effects of Surgical Masks on Cardiopulmonary Function in Healthy Subjects
Mei Li *1,2,4, Haining Ou *1,2,3,4, Qinyi Li 1,2,4, Junjie Liang 1,2,4, Weihao Liao 1,2,4, Shijuan Lang 1,2,4, Hongxin Chen 1,2,4, Yafei Wang 5, Kun Tang 1,2,4, Qiang Lin *1,2,3,4, Yuxin Zheng *1,2,4
1Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 2The Rehabilitation Medicine Lab of Guangzhou Medical University, 3Guangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Department of Rehabilitation Therapy, Guangzhou Medical University, 5Department of Rehabilitation Medicine, The Sixth Affiliated Hospital of Sun Yat-sun University

We study the effect of surgical masks on cardiopulmonary function based on a cardiopulmonary exercise test (CPET). This study shows that surgical masks reduce cardiopulmonary exercise capacity and ventilation in healthy young subjects and wearing masks might affect aerobic exercise capacity more in female subjects than in male subjects.

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Developmental Biology

Using Chicken Embryo as a Powerful Tool in Assessment of Developmental Cardiotoxicities
Qixiao Jiang 1, Xiaohui Xu 1, Jamie C. DeWitt 2, Yuxin Zheng 1
1School of Public Health, Qingdao University, 2Department of Pharmacology & Toxicology, Brody School of Medicine, East Carolina University

Chicken embryos, as a classical developmental model, are used in our lab to assess developmental cardiotoxicities following exposure to various environmental contaminants. Exposure methods and morphological/functional assessment methods established are described in this manuscript.

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Neuroscience

Motor Dual-Tasks for Gait Analysis and Evaluation in Post-Stroke Patients
Haining Ou *1,2,3,4, Shijuan Lang *1,2,3, Yuxin Zheng 1,2,3, Dongqing Huang 2, Sheng Gao 1, Meifeng Zheng 1,2, Biyi Zhao 2, Zulipiya Yiming 2, Yaxian Qiu 1,2, Qiang Lin *1,2,3,4, Junjie Liang *1,2,3
1Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 2Department of Rehabilitation Medicine, Guangzhou Medical University, 3Rehabilitation Medicine Lab, Guangzhou Medical University, 4Guangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, The Fifth Affiliated Hospital of Guangzhou Medical University

This paper presents a protocol specifically for dual motor task gait analysis in stroke patients with motor control deficits.

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Neuroscience

Dual-Task Stroop Paradigm for Detecting Cognitive Deficits in High-Functioning Stroke Patients
Songjun Lin 1,2, Qiang Lin 3,4,5, Biyi Zhao 3,4,6, Yongchun Jiang 3,4, Wanqi Zhuang 3,4, Delong Chen 3,4, Yajie Zhang 3,4, Aijia Chen 3,4, Qianrong Zhang 7, Yuxin Zheng 3,4, Jianjun Wang 1, Fangqiu Xu 8, Xi Qin 3,4,9, Yefeng Cai 10
1Department of Neurology and Psychology, the Fourth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen Traditional Chinese Medicine Hospital, 2Guangzhou University of Chinese Medicine, 3Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Department of Rehabilitation Medicine, Guangzhou Medical University, 5Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, 6School of Traditional Chinese Medicine, Jinan University, 7Health College of Guangdong Pharmaceutical University, 8Department of Clinical Medicine, Guangzhou Medical University, 9Department of Rehabilitation Medicine, Panzhihua Central Hospital, 10Department of Neurology and Psychology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Clinical assessment scales are notsensitive enough to cognitive dysfunction in high-functioning stroke patients. The dual-task paradigm presents advantages and potential in the assessment and cognitive training of cognitive dysfunction. The study here proposes a dual-task Stroop paradigm to identify cognitive dysfunction in high-functioning stroke patients.

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Neuroscience

Motor Imagery Brain-Computer Interface in Rehabilitation of Upper Limb Motor Dysfunction After Stroke
Yongchun Jiang *1,2,3, Junxiao Yin *4, Biyi Zhao 1,3,5, Yajie Zhang 1,3, Tingting Peng 1,3, Wanqi Zhuang 1,3, Siqing Wang 1,3, Siqi Huang 1,3, Meilian Zhong 1,3, Yanni Zhang 1,3, Guibing Tang 1,3, Bingchi Shen 6, Haining Ou 1,3, Yuxin Zheng *2,3, Qiang Lin *2,3
1Guangzhou Medical University, 2Department of Rehabilitation Medicine, The Seventh Affiliated Hospital of Sun Yat-sen University, 3Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Clinical Medical College of Acupuncture and Rehabilitation, Guangzhou University of Traditional Chinese Medicine, 5School of Traditional Chinese Medicine, Jinan University, 6Department of Stomatology, Second Clinical Medical College, Dongguan Campus of Guangdong Medical University

The purpose of this study is to provide an important reference for the standard clinical operation of motor imagery brain-computer interface (MI-BCI) for upper limb motor dysfunction after stroke.

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Developmental Biology

Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research
Yuefeng Li 1,2, Xiaofen Wu 1,2, Ping Hu 1,2
1Guangzhou Laboratory, 2The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University

Researchers new to the epigenetic field will find CUT&Tag a significantly easier alternative to ChIP assays. CUT&Tag has tremendously benefited the epigenetic studies on rare and primary cell populations, generating high-quality data from very few cells. This protocol describes performing H3K4me1 CUT&Tag assays on mouse myoblasts isolated from mouse hindlimb muscles.

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Neuroscience

Scalp Acupuncture Synchronizing Dual Task Gait for Enhancing Prefrontal Cortex Response
Jun Su 1, Yuanyuan Guo 2, Biyi Zhao 3, Yongchun Jiang 2,4,5, Xi Qin 6, Meilian Zhong 2,4,5, Dathan Nevin Dychingco Leung 7, Junxiao Yin 8, Qiang Lin *4, Ke Chen *9
1Department of Orthopaedics, Anshun Hospital of Traditional Chinese Medicine, 2The Fifth Affiliated Hospital of Guangzhou Medical University, 3School of Traditional Chinese Medicine, Jinan University, 4Department of Rehabilitation Medicine, The Seventh Affiliated Hospital of Sun Yat-sen University, 5Guangzhou Medical University, 6Panzhihua Central Hospital, 7Department of Physical Therapy, College of Rehabilitation Sciences, University of Santo Tomas, 8Clinical Medical College of Acupuncture and Rehabilitation, Guangzhou University of Traditional Chinese Medicine, 9Department of Spine Surgery, Zhongshan Torch Development Zone People's Hospital

This protocol demonstrates the standardized procedure of scalp acupuncture synchronizing motor-cognitive dual task and motor-cognitive dual task. This can provide an important reference for the clinical exploration of the new and effective non-drug treatment of integrated Chinese and Western medicine.

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