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Temple University

5 ARTICLES PUBLISHED IN JoVE

Medicine

Miguel G. Toscano¹, Doina Ganea¹, Ana M. Gamero²

¹Department of Microbiology and Immunology School of Medicine, Temple University , ²Department of Biochemistry, School of Medicine, Temple University

The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.

Biology

Visualization of Vascular Ca²⁺ Signaling Triggered by Paracrine Derived ROS

Karthik Mallilankaraman¹, Rajesh Kumar Gandhirajan¹, Brian J. Hawkins², Muniswamy Madesh¹

¹Department of Biochemistry, Temple University , ²Department of Anesthesiology and Pain Medicine, University of Washington

An efficient method to gain insights into visualizing the paracrine-derived ROS induction of endothelial Ca2+ signaling is described. This method takes advantage of measuring paracrine derived ROS triggered Ca2+ mobilization in vascular endothelial cells in a co-culture model.

Neuroscience

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

Andrew R. Bauder¹, Toby A. Ferguson¹

¹Center for Neural Repair and Rehabilitation, Temple University

In this report we describe a method to crush mouse sciatic nerve. This method uses readily available hemostatic forceps and easily and reproducibly produces complete sciatic nerve crush. In addition, we describe a method to prepare muscle whole mounts suitable for analysis of nerve regeneration after sciatic nerve crush.

Biology

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Conrad T. Pfeiffer¹, Christian E. Schafmeister¹

¹College of Science and Technology, Temple University

The efficient solid-phase peptide synthesis of a functionalized bis-peptide trimer utilizing a "safety catch" cleavage procedure from HMBA resin is described.

Biology

Gene Trapping Using Gal4 in Zebrafish

Jorune Balciuniene¹, Darius Balciunas¹

¹Department of Biology, Temple University

This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.