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University Hospital Tübingen

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Application of Atomic Force Microscopy to Detect Early Osteoarthritis
Marina Danalache *1, Aadhya Tiwari *1, Viktor Sigwart 1,2, Ulf Krister Hofmann 1,3
1Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, 2Medical faculty of the University of Tübingen, 3Department of Orthopaedic Surgery, University Hospital of Tübingen

We present a method to investigate early osteoarthritic changes at the cellular level in articular cartilage by using atomic force microscopy (AFM).

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Biology

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
Florian Christof Bonnaire 1,2, Martina Feierabend 3, Julius Michael Wolfgart 1,4, Wolfram Breuer 5, Christian Walter 2, Ulf Krister Hofmann 1,2, Marina Danalache 1
1Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, 2Department of Orthopedic Surgery, University Hospital of Tübingen, 3Institute for Bioinformatics and Medical Informatics, Faculty of science of the University of Tübingen, 4Medical faculty of the University of Tübingen, 5Bavarian Health and Food Safety Authority

We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.

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Bioengineering

Injection of Porcine Adipose Tissue-Derived Stroma Cells via Waterjet Technology
Jasmin Knoll *1, Marina Danalache *2, Walter Linzenbold 3, Markus Enderle 3, Tanja Abruzzese 1, Arnulf Stenzl 1, Wilhelm K. Aicher 1
1Department of Urology, University Hospital Tübingen, 2Department of Orthopaedic Surgery, University Hospital Tübingen, 3ERBE Elektromedizin GmbH Tübingen

We present a method of cell injection via needle free waterjet technology coupled with a sequela of post-delivery investigations in terms of cellular viability, proliferation, and elasticity measurements.

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Medicine

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
Wanjing Cen 1, Siegmar Reinert 1, Meltem Avci-Adali 2, Dorothea Alexander 1, Felix Umrath 1,3
1Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, 2Department of Thoracic and Cardiovascular Surgery, University Hospital Tübingen, 3Department of Orthopaedic Surgery, University Hospital Tübingen

Here, we present a simple and effective assay procedure for resorption pit assays using calcium phosphate coated cell culture plates.

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Bioengineering

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
Cyril Daniel 1, Dorothea Alexander 2, Felix Umrath 1,2, Marina Danalache 1
1Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital Tübingen, 2Department of Oral and Maxillofacial Surgery, University Hospital Tübingen

We present a step-by-step approach to identify and address the most common problems associated with atomic force microscopy micro-indentations. We exemplify the emerging problems on native human articular cartilage explants characterized by various degrees of osteoarthritis-driven degeneration.

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