University Hospital Tübingen

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Application of Atomic Force Microscopy to Detect Early Osteoarthritis

Marina Danalache^*1, Aadhya Tiwari^*1, Viktor Sigwart^1,2, Ulf Krister Hofmann^1,3

¹Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, ²Medical faculty of the University of Tübingen, ³Department of Orthopaedic Surgery, University Hospital of Tübingen

We present a method to investigate early osteoarthritic changes at the cellular level in articular cartilage by using atomic force microscopy (AFM).

Biology

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Florian Christof Bonnaire^1,2, Martina Feierabend³, Julius Michael Wolfgart^1,4, Wolfram Breuer⁵, Christian Walter², Ulf Krister Hofmann^1,2, Marina Danalache¹

¹Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, ²Department of Orthopedic Surgery, University Hospital of Tübingen, ³Institute for Bioinformatics and Medical Informatics, Faculty of science of the University of Tübingen, ⁴Medical faculty of the University of Tübingen, ⁵Bavarian Health and Food Safety Authority

We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.

Bioengineering

Injection of Porcine Adipose Tissue-Derived Stroma Cells via Waterjet Technology

Jasmin Knoll^*1, Marina Danalache^*2, Walter Linzenbold³, Markus Enderle³, Tanja Abruzzese¹, Arnulf Stenzl¹, Wilhelm K. Aicher¹

¹Department of Urology, University Hospital Tübingen, ²Department of Orthopaedic Surgery, University Hospital Tübingen, ³ERBE Elektromedizin GmbH Tübingen

We present a method of cell injection via needle free waterjet technology coupled with a sequela of post-delivery investigations in terms of cellular viability, proliferation, and elasticity measurements.

Medicine

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Wanjing Cen¹, Siegmar Reinert¹, Meltem Avci-Adali², Dorothea Alexander¹, Felix Umrath^1,3

¹Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, ²Department of Thoracic and Cardiovascular Surgery, University Hospital Tübingen, ³Department of Orthopaedic Surgery, University Hospital Tübingen

Here, we present a simple and effective assay procedure for resorption pit assays using calcium phosphate coated cell culture plates.

Bioengineering

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Cyril Daniel¹, Dorothea Alexander², Felix Umrath^1,2, Marina Danalache¹

¹Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital Tübingen, ²Department of Oral and Maxillofacial Surgery, University Hospital Tübingen

We present a step-by-step approach to identify and address the most common problems associated with atomic force microscopy micro-indentations. We exemplify the emerging problems on native human articular cartilage explants characterized by various degrees of osteoarthritis-driven degeneration.