Initiation of Independent Lineages of Sulfolobus acidocaldarius Cultures for Temperature Evolution Experimentation

To begin, use a spectrophotometer to measure the optical density of a sulfolobus acidocaldarius starting culture. Next, create serial dilutions of the starting culture from 10 to the 1 to 10 to the 6. Pipette 100 microliters each from the 10 to th