Adaptation at the Extremes of Life: Experimental Evolution with the Extremophile Archaeon Sulfolobus acidocaldarius

614 Views

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08:11 min

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June 14th, 2024

DOI :

10.3791/66271-v

June 14th, 2024

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Zahraa Al-Baqsami*¹^,²^,³, Rebecca Lowry Palmer*¹^,³, Gwyneth Darwent¹, Andrew J. McBain², Christopher G. Knight³, Danna R. Gifford¹

¹Division of Evolution, Infection and Genomics, School of Biological Sciences, The University of Manchester, ²Division of Pharmacy and Optometry, School of Health Sciences, The University of Manchester, ³Department of Earth and Environmental Sciences, School of Natural Sciences, The University of Manchester

Summary

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Experimental Evolution

Extremophile

Sulfolobus Acidocaldarius

Thermophilic Microbes

Adaptive Evolution

Cultivation Conditions

High Temperature Environments

Environmental Change

Protocol Development

High Throughput Method

Thermo Mixers

Microbial Adaptation

Energy Efficiency

Bioengineering Chassis

Low cost Equipment

Chapters in this video

0:00

Introduction

1:43

Reviving Sulfolobus acidocaldarius Cultures for Temperature Evolution Experimentation

3:14

Initiation of Independent Lineages of Sulfolobus acidocaldarius Cultures for Temperature Evolution Experimentation

4:56

Temperature Evolution Experimentation Using Sulfolobus acidocaldarius

6:42

Post‐Evolution Growth Assays of Sulfolobus acidocaldarius Ancestral and Evolved Lineages

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