October 4th, 2010
•This paper aims to instruct the reader in the operation of an integrated atomic force-optical imaging microscope for mechanical stimulation of live cells in culture. A step-by-step protocol is presented. A representative data set that shows live cell response to mechanical stimulation is presented.
Tags
Videos relacionados
Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Presynaptically Silent Synapses Studied with Light Microscopy
Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
Mechanical Stimulation of Chondrocyte-agarose Hydrogels
Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados