Name: phase contrast and differential interference contrast (dic) microscopy
Uploaded: 2008-08-06T17:41:00
Duration: 6 min 49 s
Description: Watch this Scientific Journal Video about Phase Contrast and Differential Interference Contrast DIC Microscopy at JoVE.com

Several video clips demonstrating phase contrast and DIC microscopy were taken from another video-article, entitled <a href="http://www.jove.com/details.stp?ID=197">Layers of Symbiosis</a>.&nbsp; JoVE graciously acknowledges this contribution made by the Leadbetter lab.&nbsp;

The complete text protocol for this experimental approach is available in <a href="http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/article/cb0401/current/abstract ">Current Protocols in Cell Biology.</a>

The authors have nothing to disclose.

phase contrast and differential interference contrast (dic) microscopy

Phase-contrast microscopy is often used to produce contrast for transparent, non light-absorbing, biological specimens. The technique was discovered by Zernike, in 1942, who received the Nobel prize for his achievement. DIC microscopy, introduced in the late 1960s, has been popular in biomedical research because it highlights edges of specimen structural detail, provides high-resolution optical sections of thick specimens including tissue cells, eggs, and embryos and does not suffer from the  phase halos  typical of phase-contrast images. This protocol highlights the principles and practical applications of these microscopy techniques.

Watch this Scientific Journal Video about Phase Contrast and Differential Interference Contrast DIC Microscopy at JoVE.com

Phase Contrast and Differential Interference Contrast DIC Microscopy

This protocol highlights the principles and practical applications of Phase and Differential Interference Contrast (DIC) Microscopy

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

Phase-contrast microscopy is often used to produce contrast for transparent, non light-absorbing, biological specimens. The technique was discovered by ...

Research

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