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Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica

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09:58 min

August 31st, 2011

August 31st, 2011

36,619 Views

0:05

Title

1:09

Trunk Fragment Regeneration Assay

3:00

Pharmacological Manipulation of Regeneration: Bipolarity Induced by Praziquantel

4:13

Genetic Manipulation of Regeneration: in vivo RNAi Feeding Protocol

7:54

A Standard RNAi Treatment Protocol and Representative Assay Results

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Conclusion

An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.

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