Preparation of Poly-DL-Ornithine/Laminin Coated Coverslips (Timing: 2 days)
6:18
Axon Elongation (Timing: 2 days)
7:47
Results: mES Cell Differentiation into Motor Neurons
9:12
Conclusion
We developed a new protocol to improve efficiency of in vitro differentiation of mouse embryonic stem cells into motor neurons. The differentiated ES cells acquired motor neurons features as evidenced by expression of neuronal and motor neuron markers using immunohistochemical techniques.