Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

DOI :

10.3791/50866-v

•

October 16th, 2013

•

11,865 Views

Oren Levy^1,2,3,4,5, Priya Anandakumaran^1,2,3,4,5, Jessica Ngai^1,2,3,4,5, Rohit Karnik⁶, Jeffrey M. Karp^1,2,3,4,5

¹Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, ²Center for Regenerative Therapeutics, Brigham and Women's Hospital, ³Harvard Medical School, Harvard University, ⁴Harvard Stem Cell Institute, Harvard University, ⁵Harvard-MIT Division of Health Sciences and Technology, ⁶Department of Mechanical Engineering, Massachusetts Institute of Technology

Chapters

This study used a multi-well plate microfluidic system, significantly increasing throughput of cell rolling studies under physiologically relevant shear flow. Given the importance of cell rolling in the multi-step cell homing cascade and the importance of cell homing following systemic delivery of exogenous populations of cells in patients, this system offers potential as a screening platform to improve cell-based therapy.

Tags

In Vitro Cell Rolling

Multi well Plate Microfluidic System

Cell based Therapy

Cell Homing

Vascular Endothelium

Parallel Plate Flow Chamber PPFC

Multi parameter Software Analysis Platform

Tumor Necrosis Factor alpha TNF alpha

Endothelial Cells ECs

-- Vistas

Videos relacionados

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Silk Film Culture System for in vitro Analysis and Biomaterial Design

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers