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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

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13:14 min

April 14th, 2015

April 14th, 2015

9,168 Views

0:05

Title

1:51

Preparation of Mammalian Cells, Viral Production and Transduction for Genetic Barcoding

4:59

Selection and Amplification of Genetically Barcoded Cells

6:28

Obtaining Clonal Populations of Genetically Barcoded Cells at Different Intensities

8:51

Adapt Genetically Barcoded Cell Lines to the Biolgical Application of Choice

10:52

Results: A Multiplex Analysis of Cleavage of the HIV Envelope and Dengue Virus pr-M Boundary Proteins

12:14

Conclusion

Since the discovery of the green fluorescent protein gene, fluorescent proteins have impacted molecular cell biology. This protocol describes how expression of distinct fluorescent proteins through genetic engineering is used for barcoding individual cells. The procedure enables tracking distinct populations in a cell mixture, which is ideal for multiplexed applications.

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