JoVE Logo
Centro de recursos académicos

Iniciar sesión

DNA Electroporation, Isolation and Imaging of Myofibers

DOI :

10.3791/53551-v

December 23rd, 2015

December 23rd, 2015

12,020 Views

1Center for Genetic Medicine, Northwestern University

This protocol utilizes electroporation to introduce and express fluorescently labeled proteins in mouse muscle fibers. Following recovery after electroporation, fibers are isolated. Individual fibers are then imaged using high resolution confocal microscopy to visualize muscle structure.

Tags

DNA Electroporation

-- Vistas

Videos relacionados

article

Isolation of Genomic DNA from Mouse Tails

article

Electroporation of Mycobacteria

article

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

article

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

article

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

article

Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations

article

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

article

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

article

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

article

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

JoVE Logo

Privacidad

Condiciones de uso

Políticas

Investigación

Educación

ACERCA DE JoVE

Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados