Results: Antibody Interference in HEK293 Cells and Primary Hippocampal Neurons
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Conclusion
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The overall goal of the following experiment is to study protein functions and the role of altered protein expression in an efficient and tightly controlled manner. This is achieved by applying antibodies to reduce the amount of functional protein
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Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.