Results: Quantitative Real-time PCR Evaluation of Mouse Mammary Tumors
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Conclusion
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The overall goal of this procedure is to detect the quantity of a specific MRNA from tumor cells in the lung, as a method for analyzing metastasis. The main advantage of this technique is that it is a quantitative measure of metastasis. To perform
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This protocol describes how to use quantitative Real-time PCR (QRT-PCR) to detect tumor cell specific mRNA representing metastasis within the mouse lung tissue.