Prepare Embryos for Imaging on an Upright Microscope
2:37
Wide-Field Microscopy
4:43
Confocal Microscopy
8:47
Results: Wide-field and Confocal Imaging of the Dynamics of Mitochondria and Centrosomes in Zebrafish
10:28
Conclusion
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The overall goal of this imaging method is to study subcellular organelles in a vertebrate in vivo. This method can help to answer key questions in the field of neuronal cell biology, such as dynamics of mitochondria, or centrosomes in an in-vivo
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Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.