Conventional (Tconv) and Regulatory (Treg) Splenic T Cell Phenotyping
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In Vitro Suppression Assay Using CD4+CD25+ Treg and CD8+ T Cells
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CD8+ T Cytokine Production and Cell Proliferation Analyses
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Results: Activated Treg Cell-mediated Suppression of the CD8+ Responder T Cell Response
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Conclusion
Transcribir
The overall goal of this procedure is to evaluate the in vitro suppressive function of regulatory T cells, isolated from chronic virus infected mice. This method can help answer questions about the phenotype and in vitro suppressive function of ac
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Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells.