Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
3:16
Lys-GFP BMT and Intravital Imaging Preparation
5:23
In Vivo High Resolution Mouse Calvarium Imaging
7:15
Quantitative Analysis
8:14
Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
10:10
Conclusion
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The overall goal of this procedure is to visualize and measure the in vivo engraftment of transplanted blood cells to recipient bone marrow niches. The main advantage of this technique is that it allows visualization of cellular processes like hom
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Intravital fluorescence microscopy (IVFM) of the calvarium is applied in combination with genetic animal models to study the homing and engraftment of hematopoietic cells into bone marrow (BM) niches.