Wound Healing Assay Preparation and Digital Holographic (DHM) Analysis
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Acute Dextran Sodium Sulfate (DSS) Colitis Induction and DHM Analysis
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Results: Representative DHM Analyses
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Conclusion
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The overall goal of this study is to use digital holographic microscopy or DHM to provide automated stain-free quantitative phase contrast imaging of intestinal inflammation colonic tissue samples for the multi-modal assessment of epithelial wound
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Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro.